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( a ) Number of viable cells following the association of the parasite E. histolytica with the <t>probiotic</t> E. coli Nissle, subjected to different concentrations (10 2 –10 9 ) across various time intervals, in a 96-well plate. ( b ) Impact of probiotics on the inhibition of E. histolytica trophozoites over different time periods and at varying concentrations. * p < 0.05; *** p < 0.01; **** p < 0.001; ▼ p < 0.001 Significant difference when compared to the control group.
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( a ) Number of viable cells following the association of the parasite E. histolytica with the <t>probiotic</t> E. coli Nissle, subjected to different concentrations (10 2 –10 9 ) across various time intervals, in a 96-well plate. ( b ) Impact of probiotics on the inhibition of E. histolytica trophozoites over different time periods and at varying concentrations. * p < 0.05; *** p < 0.01; **** p < 0.001; ▼ p < 0.001 Significant difference when compared to the control group.
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( a ) Number of viable cells following the association of the parasite E. histolytica with the <t>probiotic</t> E. coli Nissle, subjected to different concentrations (10 2 –10 9 ) across various time intervals, in a 96-well plate. ( b ) Impact of probiotics on the inhibition of E. histolytica trophozoites over different time periods and at varying concentrations. * p < 0.05; *** p < 0.01; **** p < 0.001; ▼ p < 0.001 Significant difference when compared to the control group.
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( a ) Number of viable cells following the association of the parasite E. histolytica with the <t>probiotic</t> E. coli Nissle, subjected to different concentrations (10 2 –10 9 ) across various time intervals, in a 96-well plate. ( b ) Impact of probiotics on the inhibition of E. histolytica trophozoites over different time periods and at varying concentrations. * p < 0.05; *** p < 0.01; **** p < 0.001; ▼ p < 0.001 Significant difference when compared to the control group.
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( a ) Number of viable cells following the association of the parasite E. histolytica with the probiotic E. coli Nissle, subjected to different concentrations (10 2 –10 9 ) across various time intervals, in a 96-well plate. ( b ) Impact of probiotics on the inhibition of E. histolytica trophozoites over different time periods and at varying concentrations. * p < 0.05; *** p < 0.01; **** p < 0.001; ▼ p < 0.001 Significant difference when compared to the control group.

Journal: Microorganisms

Article Title: Amebicidal Activity of Escherichia coli Nissle 1917 Against Entamoeba histolytica

doi: 10.3390/microorganisms13040828

Figure Lengend Snippet: ( a ) Number of viable cells following the association of the parasite E. histolytica with the probiotic E. coli Nissle, subjected to different concentrations (10 2 –10 9 ) across various time intervals, in a 96-well plate. ( b ) Impact of probiotics on the inhibition of E. histolytica trophozoites over different time periods and at varying concentrations. * p < 0.05; *** p < 0.01; **** p < 0.001; ▼ p < 0.001 Significant difference when compared to the control group.

Article Snippet: The probiotic EcN (Mutaflor; Ardeypharm, Herdecke, Germany) was acquired as a pharmaceutical product available in Canada.

Techniques: Probiotics, Inhibition, Control

ROS production evaluated by DHE and DCF fluorescence. ( a ) Quantification of DHE–DA fluorescence intensity in each group. ( b ) Fluorescence microscopic images of intracellular production of DHE–DA staining in E. histolytica after interaction with the probiotic E. coli Nissle at different concentrations over 6 h and 18 h. Bar = 50 µm. * p < 0.05; ** p < 0.002; *** p < 0.0005; **** p < 0.0001.

Journal: Microorganisms

Article Title: Amebicidal Activity of Escherichia coli Nissle 1917 Against Entamoeba histolytica

doi: 10.3390/microorganisms13040828

Figure Lengend Snippet: ROS production evaluated by DHE and DCF fluorescence. ( a ) Quantification of DHE–DA fluorescence intensity in each group. ( b ) Fluorescence microscopic images of intracellular production of DHE–DA staining in E. histolytica after interaction with the probiotic E. coli Nissle at different concentrations over 6 h and 18 h. Bar = 50 µm. * p < 0.05; ** p < 0.002; *** p < 0.0005; **** p < 0.0001.

Article Snippet: The probiotic EcN (Mutaflor; Ardeypharm, Herdecke, Germany) was acquired as a pharmaceutical product available in Canada.

Techniques: Fluorescence, Staining

ROS production evaluated by DCFH–DA fluorescence. ( a ) Quantification of DCFH–DA fluorescence intensity in each group. ( b ) Fluorescence microscopic images of intracellular production of DCFH–DA staining in E. histolytica after interaction with the probiotic E. coli Nissle at different concentrations over 6 h and 18 h. Bar = 50 µm. * p < 0.004; *** p < 0.0003; **** p < 0.0001.

Journal: Microorganisms

Article Title: Amebicidal Activity of Escherichia coli Nissle 1917 Against Entamoeba histolytica

doi: 10.3390/microorganisms13040828

Figure Lengend Snippet: ROS production evaluated by DCFH–DA fluorescence. ( a ) Quantification of DCFH–DA fluorescence intensity in each group. ( b ) Fluorescence microscopic images of intracellular production of DCFH–DA staining in E. histolytica after interaction with the probiotic E. coli Nissle at different concentrations over 6 h and 18 h. Bar = 50 µm. * p < 0.004; *** p < 0.0003; **** p < 0.0001.

Article Snippet: The probiotic EcN (Mutaflor; Ardeypharm, Herdecke, Germany) was acquired as a pharmaceutical product available in Canada.

Techniques: Fluorescence, Staining